نقش گیرنده‌های آدنوزینی A1 قشر پیریفورم بر تشنج‌های ایجاد شده به روش کیندلینگ در هیپوکمپ موش صحرایی

    Background & Aim: Hippocampal CA1 region is the most common area in the development of temporal lobe epilepsy and piriform cortex is recognized as an important structure involved in the control of kindled seizures. This fundamental study dealt with the role of adenosine A1 receptors of piriform cortex in CA1 hippocampal kindled seizures in rats. Material & Method: We implanted a tripolar electrod in the right hippocampal CA1 and two guide cannulae in the left and right piriform cortex of all the studied animals. In fully kindled animals, N6-cyclohexyladenosine (CHA a selective adenosine A1 receptors agonist injected at concentrations of 1,10 and 100 µm) and 1,3-dimethyl-8-cyclopenthylxantine(CPT a selective adenosine A1 receptors antagonist injected at concentrations of 10 and 20 µm) were microinfused into the piriform cortex and the animals were stimulated 5, 15 and 90 minutes after the drug injection. Results: Obtained data showed that CHA infusion at concentrations of 10 µm and 100 µm reduced afterdischarge duration(ADD), stage 5 seizure duration(S5D), and total seizure duration(SD). Stage 4 latency(S4L) did not change significantly. On the other hand, CPT injection at concentration of 20 µm increased ADD, S5D, SD and decreased S4L significantly. Infusion of CPT(10 µm) 5 minutes before CHA(100 µm) microinjection reduced the effects of CHA on seizure parameters. Conclusion: Thus, it can be concluded that the activity of adenosine A1 receptors in the piriform cortex has anticonvulsant effects on CA1 hippocampal kindled seizures.